<?xml version="1.0"?>
<ownershipDocument>

    <schemaVersion>X0508</schemaVersion>

    <documentType>4</documentType>

    <periodOfReport>2026-01-15</periodOfReport>

    <notSubjectToSection16>0</notSubjectToSection16>

    <issuer>
        <issuerCik>0001412665</issuerCik>
        <issuerName>MidWestOne Financial Group, Inc.</issuerName>
        <issuerTradingSymbol>MOFG</issuerTradingSymbol>
    </issuer>

    <reportingOwner>
        <reportingOwnerId>
            <rptOwnerCik>0002061385</rptOwnerCik>
            <rptOwnerName>Moore Susan M</rptOwnerName>
        </reportingOwnerId>
        <reportingOwnerAddress>
            <rptOwnerStreet1>102 S CLINTON STREET, P.O. BOX 1700</rptOwnerStreet1>
            <rptOwnerStreet2></rptOwnerStreet2>
            <rptOwnerCity>IOWA CITY</rptOwnerCity>
            <rptOwnerState>IA</rptOwnerState>
            <rptOwnerZipCode>52244-1700</rptOwnerZipCode>
            <rptOwnerStateDescription></rptOwnerStateDescription>
        </reportingOwnerAddress>
        <reportingOwnerRelationship>
            <isDirector>0</isDirector>
            <isOfficer>1</isOfficer>
            <isTenPercentOwner>0</isTenPercentOwner>
            <isOther>0</isOther>
            <officerTitle>SVP, Chief Risk Officer</officerTitle>
        </reportingOwnerRelationship>
    </reportingOwner>

    <aff10b5One>0</aff10b5One>

    <nonDerivativeTable>
        <nonDerivativeTransaction>
            <securityTitle>
                <value>Common Stock</value>
            </securityTitle>
            <transactionDate>
                <value>2026-01-15</value>
            </transactionDate>
            <transactionCoding>
                <transactionFormType>4</transactionFormType>
                <transactionCode>A</transactionCode>
                <equitySwapInvolved>0</equitySwapInvolved>
                <footnoteId id="F1"/>
            </transactionCoding>
            <transactionAmounts>
                <transactionShares>
                    <value>1125</value>
                </transactionShares>
                <transactionPricePerShare>
                    <value>0</value>
                </transactionPricePerShare>
                <transactionAcquiredDisposedCode>
                    <value>A</value>
                </transactionAcquiredDisposedCode>
            </transactionAmounts>
            <postTransactionAmounts>
                <sharesOwnedFollowingTransaction>
                    <value>11349.499</value>
                    <footnoteId id="F2"/>
                </sharesOwnedFollowingTransaction>
            </postTransactionAmounts>
            <ownershipNature>
                <directOrIndirectOwnership>
                    <value>D</value>
                </directOrIndirectOwnership>
            </ownershipNature>
        </nonDerivativeTransaction>
        <nonDerivativeHolding>
            <securityTitle>
                <value>Common Stock</value>
            </securityTitle>
            <postTransactionAmounts>
                <sharesOwnedFollowingTransaction>
                    <value>1345.938</value>
                    <footnoteId id="F3"/>
                </sharesOwnedFollowingTransaction>
            </postTransactionAmounts>
            <ownershipNature>
                <directOrIndirectOwnership>
                    <value>I</value>
                </directOrIndirectOwnership>
                <natureOfOwnership>
                    <value>By 401(k)</value>
                    <footnoteId id="F4"/>
                </natureOfOwnership>
            </ownershipNature>
        </nonDerivativeHolding>
    </nonDerivativeTable>

    <derivativeTable></derivativeTable>

    <footnotes>
        <footnote id="F1">Represents restricted stock units acquired pursuant to a grant of time-based restricted stock units which vest on January 15, 2027, or on the legal close date of the merger announced October 23, 2025, of MidWestOne Financial Group, Inc. with and into Nicolet Bankshares, Inc., with Nicolet Bankshares, Inc. as the surviving corporation, whichever comes first.</footnote>
        <footnote id="F2">Includes 9.808 Dividend Equivalents credited to unvested time-based RSUs in lieu of a cash dividend payment since the reporting person's last required Form filing. Each whole Dividend Equivalent is the economic equivalent of one share of MidWestOne Financial Group, Inc. common stock.</footnote>
        <footnote id="F3">Shares held in the reporting person's account from within the MidWestOne Financial Group, Inc. 401(k) Plan, as of December 31, 2025. Reported shares have increased by 29.394 shares since the date of the reporting person's previous Form filing due to an allocation to her account.</footnote>
        <footnote id="F4">The MidWestOne Financial Group, Inc. Employee Stock Ownership Plan (formerly the &quot;ESOP&quot;) was merged into the MidWestOne Financial Group, Inc.  401(k) Plan (the &quot;401(k)&quot;) on May 7, 2025. Shares from the reporting person's ESOP account were merged into the reporting person's account in the 401(k).</footnote>
    </footnotes>

    <remarks></remarks>

    <ownerSignature>
        <signatureName>Kenneth R. Urmie, Deputy Corporate Secretary, for Susan M. Moore under Power of Attorney dated March 3, 2025</signatureName>
        <signatureDate>2026-01-20</signatureDate>
    </ownerSignature>
</ownershipDocument>
