X0508 4 2026-01-02 0 0001999480 Alto Neuroscience, Inc. ANRO 0002008212 Cox Christopher Nixon C/O ALTO NEUROSCIENCE, INC. 650 CASTRO STREET, SUITE 450 MOUNTAIN VIEW CA 94041 1 0 0 0 0 Stock Option (right to buy) 16.59 2026-01-02 4 A 0 7189 11.82 A 2036-01-01 Common Stock 7189 7189 D 1,797 shares underlying the option shall vest on March 31, 2026, 1,797 shares underlying the option shall vest on June 30, 2026, 1,798 shares underlying the option shall vest on September 30, 2026 and 1,797 shares underlying the option shall vest on December 31, 2026, subject to the Reporting Person's continuous service through each such vesting date. This option was issued to the Reporting Person pursuant to the Issuer's Non-Employee Director Compensation Policy in lieu of retainer fees of $85,000. /s/ Erin R. McQuade, Attorney-in-Fact 2026-01-06