X0508 4 2025-12-08 0001623526 Stoke Therapeutics, Inc. STOK 0001966716 Allan Jonathan C/O STOKE THERAPEUTICS, INC. 45 WIGGINS AVENUE BEDFORD MA 01730 false true false false GENERAL COUNSEL & CORP SEC 1 Common Stock 2025-12-08 4 S 0 1300 31.5956 D 14509 D Common Stock 2025-12-08 4 S 0 2678 32.6128 D 11831 D This transaction was executed pursuant to a Rule 10b5-1 trading plan adopted by the reporting person on December 12, 2024. The reported price in Column 4 is a weighted average price. These shares were sold in multiple transactions at prices ranging from $31.27 to $32.14 per share, inclusive. The reporting person undertakes to provide to the Issuer, any security holder of the Issuer, or the staff of the Securities and Exchange Commission, upon request, full information regarding the number of shares sold at each separate price within the ranges set forth in this footnote and in footnote 3 of this Form 4. The price reported in Column 4 is a weighted average price. These shares were sold in multiple transactions at prices ranging from $32.29 to $33.19 per share, inclusive. /s/ Jonathan Allan 2025-12-10