X0508 4 2025-12-03 0001623526 Stoke Therapeutics, Inc. STOK 0001966716 Allan Jonathan C/O STOKE THERAPEUTICS, INC. 45 WIGGINS AVENUE BEDFORD MA 01730 false true false false GENERAL COUNSEL & CORP SEC 1 Common Stock 2025-12-03 4 M 0 6000 0 A 25612 D Common Stock 2025-12-04 4 S 0 824 30.7635 D 24788 D Common Stock 2025-12-04 4 S 0 194 31.3164 D 24594 D Common Stock 2025-12-05 4 S 0 1004 31.3304 D 23590 D Common Stock 2025-12-05 4 S 0 5437 31.0828 D 18153 D Common Stock 2025-12-05 4 S 0 2344 31.7597 D 15809 D Performance Stock Units 2025-12-03 4 M 0 6000 0 D 2025-12-03 Common Stock 6000 0 D The reported transaction represents an Issuer mandated sale to satisfy tax withholding liabilities in connection with the vesting and settlement of restricted stock units. The reported price in Column 4 is a weighted average price. These shares were sold in multiple transactions at prices ranging from $30.23 to $31.19 per share, inclusive. The reporting person undertakes to provide to the Issuer, any security holder of the Issuer, or the staff of the Securities and Exchange Commission, upon request, full information regarding the number of shares sold at each separate price within the ranges set forth in this footnote and in footnotes 3, 4, 6, and 7 of this Form 4. The price reported in Column 4 is a weighted average price. These shares were sold in multiple transactions at prices ranging from $31.23 to $31.505 per share, inclusive. The reported price in Column 4 is a weighted average price. These shares were sold in multiple transactions at prices ranging from $30.93 to $31.69 per share, inclusive. This transaction was executed pursuant to a Rule 10b5-1 trading plan adopted by the reporting person on December 12, 2024. The price reported in Column 4 is a weighted average price. These shares were sold in multiple transactions at prices ranging from $30.58 to $31.57 per share, inclusive. The price reported in Column 4 is a weighted average price. These shares were sold in multiple transactions at prices ranging from $31.59 to $31.97 per share, inclusive. Each restricted stock unit represents a contingent right to receive one share of the Issuer's Common Stock upon settlement. Half the award vested on December 3, 2024 and the remainder vested or vests on December 3, 2025, subject to the reporting person's continued service to the Issuer on the relevant vesting date. /s/ Jonathan Allan 2025-12-05