X0508 4 2025-12-01 0001623526 Stoke Therapeutics, Inc. STOK 0001780088 Ticho Barry C/O STOKE THERAPEUTICS, INC. 45 WIGGINS AVENUE BEDFORD MA 01730 false true false false CHIEF MEDICAL OFFICER 0 Common Stock 2025-12-01 4 M 0 11981 0 A 19176 D Common Stock 2025-12-02 4 S 0 1244 29.5620 D 17932 D Common Stock 2025-12-02 4 S 0 762 30.1419 D 17170 D Common Stock 2025-12-03 4 S 0 789 30.3380 D 16381 D Common Stock 2025-12-03 4 S 0 1225 30.8477 D 15156 D Restricted Stock Units 2025-12-01 4 M 0 11981 0 D 2025-12-01 Common Stock 11981 0 D The reported transaction represents an Issuer mandated sale to satisfy tax withholding liabilities in connection with the vesting and settlement of restricted stock units. The price reported in Column 4 is a weighted average price. These shares were sold in multiple transactions at prices ranging from $28.93 to $29.92 per share, inclusive. The reporting person undertakes to provide to the Issuer, any security holder of the Issuer, or the staff of the Securities and Exchange Commission, upon request, full information regarding the number of shares sold at each separate price within the ranges set forth in this footnote and in footnotes 3 through 5. The reported price in Column 4 is a weighted average price. These shares were sold in multiple transactions at prices ranging from $29.93 to $30.63 per share, inclusive. The reported price in Column 4 is a weighted average price. These shares were sold in multiple transactions at prices ranging from $29.81 to $30.805 per share, inclusive. The reported price in Column 4 is a weighted average price. These shares were sold in multiple transactions at prices ranging from $30.815 to $31.17 per share, inclusive. Each restricted stock unit represents a contingent right to receive one share of the Issuer's Common Stock upon settlement. 48,792 restricted stock units vested on December 1, 2024 and 11,981 vest on December 1, 2025, subject to the reporting person's continued service to the Issuer on each such date. /s/ Jonathan Allan, Attorney-in-Fact 2025-12-03