<?xml version="1.0"?>
<ownershipDocument>

    <schemaVersion>X0508</schemaVersion>

    <documentType>4</documentType>

    <periodOfReport>2026-01-15</periodOfReport>

    <notSubjectToSection16>0</notSubjectToSection16>

    <issuer>
        <issuerCik>0001412665</issuerCik>
        <issuerName>MidWestOne Financial Group, Inc.</issuerName>
        <issuerTradingSymbol>MOFG</issuerTradingSymbol>
    </issuer>

    <reportingOwner>
        <reportingOwnerId>
            <rptOwnerCik>0001769530</rptOwnerCik>
            <rptOwnerName>Ho-Sing-Loy Paul A</rptOwnerName>
        </reportingOwnerId>
        <reportingOwnerAddress>
            <rptOwnerStreet1>102 S CLINTON STREET</rptOwnerStreet1>
            <rptOwnerStreet2>P.O. BOX 1700</rptOwnerStreet2>
            <rptOwnerCity>IOWA CITY</rptOwnerCity>
            <rptOwnerState>IA</rptOwnerState>
            <rptOwnerZipCode>52244-1700</rptOwnerZipCode>
            <rptOwnerStateDescription></rptOwnerStateDescription>
        </reportingOwnerAddress>
        <reportingOwnerRelationship>
            <isDirector>0</isDirector>
            <isOfficer>1</isOfficer>
            <isTenPercentOwner>0</isTenPercentOwner>
            <isOther>0</isOther>
            <officerTitle>VP, Chief Information Officer</officerTitle>
        </reportingOwnerRelationship>
    </reportingOwner>

    <aff10b5One>0</aff10b5One>

    <nonDerivativeTable>
        <nonDerivativeTransaction>
            <securityTitle>
                <value>Common Stock</value>
            </securityTitle>
            <transactionDate>
                <value>2026-01-15</value>
            </transactionDate>
            <transactionCoding>
                <transactionFormType>4</transactionFormType>
                <transactionCode>A</transactionCode>
                <equitySwapInvolved>0</equitySwapInvolved>
                <footnoteId id="F1"/>
            </transactionCoding>
            <transactionAmounts>
                <transactionShares>
                    <value>1243</value>
                </transactionShares>
                <transactionPricePerShare>
                    <value>0</value>
                </transactionPricePerShare>
                <transactionAcquiredDisposedCode>
                    <value>A</value>
                </transactionAcquiredDisposedCode>
            </transactionAmounts>
            <postTransactionAmounts>
                <sharesOwnedFollowingTransaction>
                    <value>6523</value>
                </sharesOwnedFollowingTransaction>
            </postTransactionAmounts>
            <ownershipNature>
                <directOrIndirectOwnership>
                    <value>D</value>
                </directOrIndirectOwnership>
            </ownershipNature>
        </nonDerivativeTransaction>
    </nonDerivativeTable>

    <derivativeTable></derivativeTable>

    <footnotes>
        <footnote id="F1">Represents restricted stock units acquired pursuant to a grant of time-based restricted stock units which vest on January 15, 2027, or on the legal close date of the merger announced October 23, 2025, of MidWestOne Financial Group, Inc. with and into Nicolet Bankshares, Inc., with Nicolet Bankshares, Inc. as the surviving corporation, whichever comes first.</footnote>
    </footnotes>

    <remarks></remarks>

    <ownerSignature>
        <signatureName>Kenneth R. Urmie, Deputy Corporate Secretary, for Paul A. Ho-Sing-Loy under Power of Attorney dated March 2, 2025</signatureName>
        <signatureDate>2026-01-16</signatureDate>
    </ownerSignature>
</ownershipDocument>
