X0508 4 2026-03-10 0001623526 Stoke Therapeutics, Inc. STOK 0001586226 TZIANABOS ARTHUR C/O STOKE THERAPEUTICS, INC. 45 WIGGINS AVENUE BEDFORD MA 01730 true true false false Interim Executive Chair 1 Common Stock 2026-03-10 4 M 0 3955 8.33 A 35694 D Common Stock 2026-03-10 4 S 0 4355 40.0033 D 31339 D Stock Option (Right to Buy) 8.33 2026-03-10 4 M 0 3955 0 D 2034-03-19 Common Stock 3955 118696 D The transaction reported on this Form 4 was executed pursuant to a Rule 10b5-1 trading plan adopted by the reporting person on August 15, 2025. The reported price in Column 4 is a weighted average price. These shares were sold in multiple transactions at prices ranging from $40.00 to $40.095 per share, inclusive. The reporting person undertakes to provide to the Issuer, any security holder of the Issuer, or the staff of the Securities and Exchange Commission, upon request, full information regarding the number of shares sold at each separate price within the range set forth in this footnote. Effective October 6, 2025, this option became fully vested and exercisable. /s/ Jonathan Allan, Attorney-in-Fact 2026-03-12