X0508 4 2026-03-04 0 0001808805 Nautilus Biotechnology, Inc. NAUT 0001378830 Patel Sujal M C/O NAUTILUS BIOTECHNOLOGY, INC. 2701 EASTLAKE AVENUE EAST SEATTLE WA 98102 1 1 1 0 CEO, President, and Secretary 0 Common Stock 2026-03-04 4 P 0 25000 2.6095 A 10366721 D Common Stock 5280476 I By PFV I, LLC Common Stock 1814035 I By Sujal Patel 2020 Children's Trust, u/a/d December 3, 2020 Represents the weighted average share price of an aggregate total of 25,000 shares purchased in the price range of $2.48 to $2.6095 by the reporting person. The reporting person undertakes to provide upon request by the Commission staff, the issuer or a security holder of the issuer, full information regarding the number of shares purchased at each separate price. Shares held by PFV I, LLC. The reporting person is the manager of PFV I, LLC. Shares held by Sujal Patel 2020 Children's Trust, u/a/d December 3, 2020 (the "Patel Trust"). The reporting person and the reporting person's spouse are the trustees of the Patel Trust. /s/ Matthew Murphy, as Attorney-in-Fact 2026-03-04