X0609 4 2026-09-15 0001863127 Tyra Biosciences, Inc. TYRA 0001346824 RA CAPITAL MANAGEMENT, L.P. false 200 BERKELEY STREET, 18TH FLOOR BOSTON MA 02116 true false true false 0001315082 RA Capital Healthcare Fund LP false 200 BERKELEY STREET, 18TH FLOOR BOSTON MA 02116 1 0 1 0 0001780117 RA Capital Nexus Fund, L.P. false 200 BERKELEY STREET, 18TH FLOOR BOSTON MA 02116 1 0 0 0 0001384859 Kolchinsky Peter false C/O RA CAPITAL MANAGEMENT, L.P. 200 BERKELEY STREET, 18TH FLOOR BOSTON MA 02116 1 0 1 0 0001619841 Shah Rajeev M. false C/O RA CAPITAL MANAGEMENT, L.P. 200 BERKELEY STREET, 18TH FLOOR BOSTON MA 02116 1 0 1 0 0 Pre-Funded Warrants (Right to Buy) 0.001 2026-09-15 4 P 0 9078529 22.029 A Common Stock 9078529 11616986 I See footnotes The Pre-Funded Warrants have no expiration date and are exercisable immediately. Notwithstanding the foregoing, the Reporting Persons shall not be entitled to exercise the Pre-Funded Warrants to the extent that it would cause the aggregate number of shares of Common Stock beneficially owned by the Reporting Persons, their affiliates and any persons who are members of a Section 13(d) group with the Reporting Persons or their affiliates to exceed 19.99% of the total number of issued and outstanding shares of Common Stock of the Issuer following such exercise. RA Capital Management, L.P. (the "Adviser") is the investment manager for RA Capital Healthcare Fund, L.P. (the "Fund"), RA Capital Nexus Fund, L.P. (the "Nexus Fund"), and a separately managed account (the "Account"). The general partner of the Adviser is RA Capital Management GP, LLC (the "Adviser GP"), of which Dr. Peter Kolchinsky and Mr. Rajeev Shah are the managing members. The Adviser, the Adviser GP, Dr. Kolchinsky, and Mr. Shah disclaim beneficial ownership of any of the reported securities, except to the extent of their pecuniary interest therein. Held directly by the Fund. Jake Simson, a Partner of the Adviser, serves on the Issuer's board of directors. /s/ Peter Kolchinsky, Manager of RA Capital Management, L.P. 2026-09-17 /s/ Peter Kolchinsky, Manager of RA Capital Healthcare Fund GP, LLC the General Partner of RA Capital Healthcare Fund, L.P. 2026-09-17 /s/ Peter Kolchinsky, Manager of RA Capital Nexus Fund GP, LLC the General Partner of RA Capital Nexus Fund, L.P. 2026-09-17 /s/ Peter Kolchinsky, individually 2026-09-17 /s/ Rajeev Shah, individually 2026-09-17