X0609 4 2026-09-21 0001581280 Twist Bioscience Corp TWST 0001883451 Cho Dennis false C/O TWIST BIOSCIENCE CORPORATION 681 GATEWAY BLVD SOUTH SAN FRANCISCO CA 94080 false true false false See Remarks 0 Common Stock 2026-09-21 4 S false 348 165.491 D 73303 D Represents the number of shares required to be sold by the Reporting Person to cover tax withholding obligations in connection with the vesting of Restricted Stock Units. These sales are mandated by the Issuer's election under its equity incentive plans to require the satisfaction of a tax withholding obligation to be funded by a "sell to cover" transaction and do not represent discretionary trades by the Reporting Person. Senior Vice President, Chief Legal Officer & Corporate Secretary /s/ Kendra Fox, as Attorney-in-Fact for Dennis Cho 2026-09-23