<?xml version="1.0"?>
<ownershipDocument>

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    <documentType>4</documentType>

    <periodOfReport>2026-04-16</periodOfReport>

    <notSubjectToSection16>true</notSubjectToSection16>

    <issuer>
        <issuerCik>0002104204</issuerCik>
        <issuerName>Alamar Biosciences, Inc.</issuerName>
        <issuerTradingSymbol>ALMR</issuerTradingSymbol>
        <issuerForeignTradingSymbol></issuerForeignTradingSymbol>
    </issuer>

    <reportingOwner>
        <reportingOwnerId>
            <rptOwnerCik>0001779219</rptOwnerCik>
            <rptOwnerName>Illumina Innovation Fund II GP, L.L.C.</rptOwnerName>
        </reportingOwnerId>
        <reportingOwnerAddress>
            <rptOwnerNonUSAddressFlag>false</rptOwnerNonUSAddressFlag>
            <rptOwnerStreet1>500 LINCOLN CENTRE DRIVE, SUITE 110</rptOwnerStreet1>
            <rptOwnerStreet2></rptOwnerStreet2>
            <rptOwnerCity>FOSTER CITY</rptOwnerCity>
            <rptOwnerState>CA</rptOwnerState>
            <rptOwnerZipCode>94404</rptOwnerZipCode>
            <rptOwnerStateDescription></rptOwnerStateDescription>
        </reportingOwnerAddress>
        <reportingOwnerRelationship>
            <isDirector>false</isDirector>
            <isOfficer>false</isOfficer>
            <isTenPercentOwner>true</isTenPercentOwner>
            <isOther>false</isOther>
        </reportingOwnerRelationship>
    </reportingOwner>

    <reportingOwner>
        <reportingOwnerId>
            <rptOwnerCik>0001779218</rptOwnerCik>
            <rptOwnerName>Illumina Innovation Fund II, L.P.</rptOwnerName>
        </reportingOwnerId>
        <reportingOwnerAddress>
            <rptOwnerNonUSAddressFlag>false</rptOwnerNonUSAddressFlag>
            <rptOwnerStreet1>500 LINCOLN CENTRE DRIVE</rptOwnerStreet1>
            <rptOwnerStreet2>SUITE 110</rptOwnerStreet2>
            <rptOwnerCity>FOSTER CITY</rptOwnerCity>
            <rptOwnerState>CA</rptOwnerState>
            <rptOwnerZipCode>94404</rptOwnerZipCode>
            <rptOwnerStateDescription></rptOwnerStateDescription>
        </reportingOwnerAddress>
        <reportingOwnerRelationship>
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            <isOfficer>false</isOfficer>
            <isTenPercentOwner>true</isTenPercentOwner>
            <isOther>false</isOther>
        </reportingOwnerRelationship>
    </reportingOwner>

    <reportingOwner>
        <reportingOwnerId>
            <rptOwnerCik>0002032563</rptOwnerCik>
            <rptOwnerName>Illumina Innovation Fund III GP, L.L.C.</rptOwnerName>
        </reportingOwnerId>
        <reportingOwnerAddress>
            <rptOwnerNonUSAddressFlag>false</rptOwnerNonUSAddressFlag>
            <rptOwnerStreet1>500 LINCOLN CENTRE DRIVE</rptOwnerStreet1>
            <rptOwnerStreet2>SUITE 110</rptOwnerStreet2>
            <rptOwnerCity>FOSTER CITY</rptOwnerCity>
            <rptOwnerState>CA</rptOwnerState>
            <rptOwnerZipCode>94404</rptOwnerZipCode>
            <rptOwnerStateDescription></rptOwnerStateDescription>
        </reportingOwnerAddress>
        <reportingOwnerRelationship>
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            <isTenPercentOwner>true</isTenPercentOwner>
            <isOther>false</isOther>
        </reportingOwnerRelationship>
    </reportingOwner>

    <reportingOwner>
        <reportingOwnerId>
            <rptOwnerCik>0002032562</rptOwnerCik>
            <rptOwnerName>Illumina Innovation Fund III, L.P.</rptOwnerName>
        </reportingOwnerId>
        <reportingOwnerAddress>
            <rptOwnerNonUSAddressFlag>false</rptOwnerNonUSAddressFlag>
            <rptOwnerStreet1>500 LINCOLN CENTRE DRIVE</rptOwnerStreet1>
            <rptOwnerStreet2>SUITE 110</rptOwnerStreet2>
            <rptOwnerCity>FOSTER CITY</rptOwnerCity>
            <rptOwnerState>CA</rptOwnerState>
            <rptOwnerZipCode>94404</rptOwnerZipCode>
            <rptOwnerStateDescription></rptOwnerStateDescription>
        </reportingOwnerAddress>
        <reportingOwnerRelationship>
            <isDirector>false</isDirector>
            <isOfficer>false</isOfficer>
            <isTenPercentOwner>true</isTenPercentOwner>
            <isOther>false</isOther>
        </reportingOwnerRelationship>
    </reportingOwner>

    <reportingOwner>
        <reportingOwnerId>
            <rptOwnerCik>0001534166</rptOwnerCik>
            <rptOwnerName>Naclerio Nicholas</rptOwnerName>
        </reportingOwnerId>
        <reportingOwnerAddress>
            <rptOwnerNonUSAddressFlag>false</rptOwnerNonUSAddressFlag>
            <rptOwnerStreet1>500 LINCOLN CENTRE DRIVE</rptOwnerStreet1>
            <rptOwnerStreet2>SUITE 110</rptOwnerStreet2>
            <rptOwnerCity>FOSTER CITY</rptOwnerCity>
            <rptOwnerState>CA</rptOwnerState>
            <rptOwnerZipCode>94404</rptOwnerZipCode>
            <rptOwnerStateDescription></rptOwnerStateDescription>
        </reportingOwnerAddress>
        <reportingOwnerRelationship>
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            <isOfficer>false</isOfficer>
            <isTenPercentOwner>true</isTenPercentOwner>
            <isOther>false</isOther>
        </reportingOwnerRelationship>
    </reportingOwner>

    <aff10b5One>false</aff10b5One>

    <nonDerivativeTable>
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            </securityTitle>
            <transactionDate>
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            </transactionDate>
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                <transactionFormType>4</transactionFormType>
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                </transactionAcquiredDisposedCode>
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                </sharesOwnedFollowingTransaction>
            </postTransactionAmounts>
            <ownershipNature>
                <directOrIndirectOwnership>
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                <natureOfOwnership>
                    <value>See footnote</value>
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                </natureOfOwnership>
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            </postTransactionAmounts>
            <ownershipNature>
                <directOrIndirectOwnership>
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                </directOrIndirectOwnership>
                <natureOfOwnership>
                    <value>See footnote</value>
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                </natureOfOwnership>
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        </nonDerivativeTransaction>
        <nonDerivativeTransaction>
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            <ownershipNature>
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                </directOrIndirectOwnership>
                <natureOfOwnership>
                    <value>See footnote</value>
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            </postTransactionAmounts>
            <ownershipNature>
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                <natureOfOwnership>
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                </natureOfOwnership>
            </ownershipNature>
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                <value>Common Stock</value>
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            </transactionDate>
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                <equitySwapInvolved>false</equitySwapInvolved>
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                <sharesOwnedFollowingTransaction>
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                </sharesOwnedFollowingTransaction>
            </postTransactionAmounts>
            <ownershipNature>
                <directOrIndirectOwnership>
                    <value>I</value>
                </directOrIndirectOwnership>
                <natureOfOwnership>
                    <value>See footnote</value>
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                </natureOfOwnership>
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        </nonDerivativeTransaction>
        <nonDerivativeTransaction>
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                <equitySwapInvolved>false</equitySwapInvolved>
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                </sharesOwnedFollowingTransaction>
            </postTransactionAmounts>
            <ownershipNature>
                <directOrIndirectOwnership>
                    <value>I</value>
                </directOrIndirectOwnership>
                <natureOfOwnership>
                    <value>See footnote</value>
                    <footnoteId id="F6"/>
                </natureOfOwnership>
            </ownershipNature>
        </nonDerivativeTransaction>
        <nonDerivativeTransaction>
            <securityTitle>
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            </transactionDate>
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                <transactionCode>P</transactionCode>
                <equitySwapInvolved>false</equitySwapInvolved>
            </transactionCoding>
            <transactionAmounts>
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                </transactionShares>
                <transactionPricePerShare>
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                </transactionPricePerShare>
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                </transactionAcquiredDisposedCode>
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                </sharesOwnedFollowingTransaction>
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                <directOrIndirectOwnership>
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                <transactionPricePerShare>
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                </underlyingSecurityShares>
            </underlyingSecurity>
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            </postTransactionAmounts>
            <ownershipNature>
                <directOrIndirectOwnership>
                    <value>I</value>
                </directOrIndirectOwnership>
                <natureOfOwnership>
                    <value>See Footnote</value>
                    <footnoteId id="F2"/>
                </natureOfOwnership>
            </ownershipNature>
        </derivativeTransaction>
        <derivativeTransaction>
            <securityTitle>
                <value>Series A-4 Preferred Stock</value>
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                <footnoteId id="F1"/>
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            <transactionDate>
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                <equitySwapInvolved>false</equitySwapInvolved>
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                </underlyingSecurityTitle>
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                </underlyingSecurityShares>
            </underlyingSecurity>
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            </postTransactionAmounts>
            <ownershipNature>
                <directOrIndirectOwnership>
                    <value>I</value>
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                <natureOfOwnership>
                    <value>See Footnote</value>
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        </derivativeTransaction>
        <derivativeTransaction>
            <securityTitle>
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                <transactionShares>
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                <transactionPricePerShare>
                    <value>0</value>
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                </underlyingSecurityShares>
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            <ownershipNature>
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                <natureOfOwnership>
                    <value>See Footnote</value>
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            </ownershipNature>
        </derivativeTransaction>
        <derivativeTransaction>
            <securityTitle>
                <value>Series C Preferred Stock</value>
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                <transactionPricePerShare>
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                <footnoteId id="F1"/>
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                </underlyingSecurityShares>
            </underlyingSecurity>
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            <ownershipNature>
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                <natureOfOwnership>
                    <value>See Footnote</value>
                    <footnoteId id="F2"/>
                </natureOfOwnership>
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        </derivativeTransaction>
        <derivativeTransaction>
            <securityTitle>
                <value>Convertible Promissory Note</value>
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            <transactionDate>
                <value>2026-04-20</value>
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                <transactionCode>C</transactionCode>
                <equitySwapInvolved>false</equitySwapInvolved>
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            <transactionAmounts>
                <transactionTotalValue>
                    <value>5000000</value>
                </transactionTotalValue>
                <transactionPricePerShare>
                    <value>0</value>
                </transactionPricePerShare>
                <transactionAcquiredDisposedCode>
                    <value>D</value>
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            <exerciseDate>
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            <expirationDate>
                <footnoteId id="F5"/>
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                    <value>I</value>
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                <natureOfOwnership>
                    <value>See Footnote</value>
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        <derivativeTransaction>
            <securityTitle>
                <value>Convertible Promissory Note</value>
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                <value>2026-04-20</value>
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                <transactionCode>C</transactionCode>
                <equitySwapInvolved>false</equitySwapInvolved>
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                <transactionTotalValue>
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                <transactionPricePerShare>
                    <value>0</value>
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                <transactionAcquiredDisposedCode>
                    <value>D</value>
                </transactionAcquiredDisposedCode>
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            <exerciseDate>
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            <expirationDate>
                <footnoteId id="F5"/>
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                    <value>Common Stock</value>
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                    <value>0</value>
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            <ownershipNature>
                <directOrIndirectOwnership>
                    <value>I</value>
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                <natureOfOwnership>
                    <value>See Footnote</value>
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        </derivativeTransaction>
    </derivativeTable>

    <footnotes>
        <footnote id="F1">The Series A-3 Preferred Stock, Series A-4 Preferred Stock and Series C Preferred Stock are convertible into shares of Class B Common Stock on a 1-for-2.418 basis and have no expiration date. The Series A-3 Preferred Stock, Series A-4 Preferred Stock and Series C Preferred Stock automatically converted into shares of Class B Common Stock in connection with the closing of the initial public offering of the Issuer's Common Stock (the &quot;IPO&quot;).</footnote>
        <footnote id="F2">The securities are held by Illumina Innovation Fund II, L.P. (&quot;IIF II&quot;). Illumina Innovation Fund II GP, L.L.C. (&quot;IIF II GP&quot;) is the general partner of IIF II. Nicholas Naclerio (&quot;Mr. Naclerio&quot;), a member of the Issuer's board of directors, is the sole managing member of IIF II GP and may be deemed to have voting, investment and dispositive power with respect to the securities held by IIF II. Each of IIF II GP and Mr. Naclerio disclaim beneficial ownership over the securities held by IIF II, except to the extent of their respective pecuniary interests therein, if any.</footnote>
        <footnote id="F3">The Series B Preferred Stock is convertible into shares of Class B Common Stock on a 1-for-2.271 basis and has no expiration date. The Series B Preferred Stock automatically converted into shares of Class B Common Stock in connection with the closing of the IPO.</footnote>
        <footnote id="F4">Each share of Class B Common Stock was reclassified into one share of Common Stock immediately prior to the completion of the IPO.</footnote>
        <footnote id="F5">Reflects a convertible note that is convertible into shares of Common Stock of the Issuer. The convertible note has a maturity date of July 8, 2027. The principal amount of the convertible note was automatically converted into shares of Common Stock of the Issuer upon the closing of the Issuer's IPO at a conversion price equal to the initial public offering price of the Issuer's Common Stock multiplied by 0.85.</footnote>
        <footnote id="F6">The securities are held by Illumina Innovation Fund III, L.P. (&quot;IIF III&quot;). Illumina Innovation Fund III GP, L.L.C. (&quot;IIF III GP&quot;) is the general partner of IIF III. Mr. Naclerio, a member of the Issuer's board of directors, is the sole managing member of IIF III GP and may be deemed to have voting, investment and dispositive power with respect to the securities held by IIF III. Each of IIF III GP and Mr. Naclerio disclaim beneficial ownership over the securities held by IIF III, except to the extent of their respective pecuniary interests therein, if any.</footnote>
    </footnotes>

    <ownerSignature>
        <signatureName>/s/ Nicholas Naclerio, Managing Member of Illumina Innovation Fund II GP, L.L.C.</signatureName>
        <signatureDate>2026-04-20</signatureDate>
    </ownerSignature>

    <ownerSignature>
        <signatureName>/s/ Nicholas Naclerio, Managing Member of Illumina Innovation Fund II GP, L.L.C., the general partner of Illumina Innovation Fund II, L.P.</signatureName>
        <signatureDate>2026-04-20</signatureDate>
    </ownerSignature>

    <ownerSignature>
        <signatureName>/s/ Nicholas Naclerio, Managing Member of Illumina Innovation Fund III GP, L.L.C.</signatureName>
        <signatureDate>2026-04-20</signatureDate>
    </ownerSignature>

    <ownerSignature>
        <signatureName>/s/ Nicholas Naclerio, Managing Member of Illumina Innovation Fund III GP, L.L.C., the general partner of Illumina Innovation Fund III, L.P.</signatureName>
        <signatureDate>2026-04-20</signatureDate>
    </ownerSignature>

    <ownerSignature>
        <signatureName>/s/ Nicholas Naclerio</signatureName>
        <signatureDate>2026-04-20</signatureDate>
    </ownerSignature>
</ownershipDocument>
