<?xml version="1.0"?>
<ownershipDocument>

    <schemaVersion>X0508</schemaVersion>

    <documentType>4</documentType>

    <periodOfReport>2026-01-05</periodOfReport>

    <issuer>
        <issuerCik>0001861795</issuerCik>
        <issuerName>Definitive Healthcare Corp.</issuerName>
        <issuerTradingSymbol>DH</issuerTradingSymbol>
    </issuer>

    <reportingOwner>
        <reportingOwnerId>
            <rptOwnerCik>0002052043</rptOwnerCik>
            <rptOwnerName>Heller Casey</rptOwnerName>
        </reportingOwnerId>
        <reportingOwnerAddress>
            <rptOwnerStreet1>C/O DEFINITIVE HEALTHCARE CORP.</rptOwnerStreet1>
            <rptOwnerStreet2>492 OLD CONNECTICUT PATH, SUITE 401</rptOwnerStreet2>
            <rptOwnerCity>FRAMINGHAM</rptOwnerCity>
            <rptOwnerState>MA</rptOwnerState>
            <rptOwnerZipCode>01701</rptOwnerZipCode>
            <rptOwnerStateDescription></rptOwnerStateDescription>
        </reportingOwnerAddress>
        <reportingOwnerRelationship>
            <isDirector>false</isDirector>
            <isOfficer>true</isOfficer>
            <isTenPercentOwner>false</isTenPercentOwner>
            <isOther>false</isOther>
            <officerTitle>Chief Financial Officer</officerTitle>
        </reportingOwnerRelationship>
    </reportingOwner>

    <aff10b5One>false</aff10b5One>

    <nonDerivativeTable>
        <nonDerivativeTransaction>
            <securityTitle>
                <value>Class A Common Stock</value>
            </securityTitle>
            <transactionDate>
                <value>2026-01-05</value>
            </transactionDate>
            <transactionCoding>
                <transactionFormType>4</transactionFormType>
                <transactionCode>A</transactionCode>
                <equitySwapInvolved>false</equitySwapInvolved>
            </transactionCoding>
            <transactionAmounts>
                <transactionShares>
                    <value>195313</value>
                    <footnoteId id="F1"/>
                </transactionShares>
                <transactionPricePerShare>
                    <value>0</value>
                </transactionPricePerShare>
                <transactionAcquiredDisposedCode>
                    <value>A</value>
                </transactionAcquiredDisposedCode>
            </transactionAmounts>
            <postTransactionAmounts>
                <sharesOwnedFollowingTransaction>
                    <value>1275490</value>
                </sharesOwnedFollowingTransaction>
            </postTransactionAmounts>
            <ownershipNature>
                <directOrIndirectOwnership>
                    <value>D</value>
                </directOrIndirectOwnership>
            </ownershipNature>
        </nonDerivativeTransaction>
    </nonDerivativeTable>

    <footnotes>
        <footnote id="F1">Represents restricted stock units (&quot;RSUs&quot;), each representing a contingent right to receive one share of the Issuer's Class A Common Stock, par value $0.001 (the &quot;Class A Common Stock&quot;), with 50% of the RSUs vesting on January 1, 2027, followed by vesting in equal quarterly installments over the remaining 12 months thereafter, subject to the Reporting Person's continued service with the Issuer through each such vesting date.</footnote>
    </footnotes>

    <ownerSignature>
        <signatureName>/s/ Jonathan Paris, Attorney-in-Fact</signatureName>
        <signatureDate>2026-01-07</signatureDate>
    </ownerSignature>
</ownershipDocument>
