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<ownershipDocument>

    <schemaVersion>X0508</schemaVersion>

    <documentType>4</documentType>

    <periodOfReport>2025-12-12</periodOfReport>

    <issuer>
        <issuerCik>0001817241</issuerCik>
        <issuerName>Artiva Biotherapeutics, Inc.</issuerName>
        <issuerTradingSymbol>ARTV</issuerTradingSymbol>
    </issuer>

    <reportingOwner>
        <reportingOwnerId>
            <rptOwnerCik>0002028466</rptOwnerCik>
            <rptOwnerName>Raymon Heather</rptOwnerName>
        </reportingOwnerId>
        <reportingOwnerAddress>
            <rptOwnerStreet1>C/O ARTIVA BIOTHERAPEUTICS, INC.</rptOwnerStreet1>
            <rptOwnerStreet2>5505 MOREHOUSE DRIVE, SUITE 100</rptOwnerStreet2>
            <rptOwnerCity>SAN DIEGO</rptOwnerCity>
            <rptOwnerState>CA</rptOwnerState>
            <rptOwnerZipCode>92121</rptOwnerZipCode>
            <rptOwnerStateDescription></rptOwnerStateDescription>
        </reportingOwnerAddress>
        <reportingOwnerRelationship>
            <isDirector>false</isDirector>
            <isOfficer>true</isOfficer>
            <isTenPercentOwner>false</isTenPercentOwner>
            <isOther>false</isOther>
            <officerTitle>SVP, Research and Development</officerTitle>
        </reportingOwnerRelationship>
    </reportingOwner>

    <aff10b5One>false</aff10b5One>

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                <equitySwapInvolved>false</equitySwapInvolved>
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                </transactionShares>
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            </transactionDate>
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                <equitySwapInvolved>false</equitySwapInvolved>
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                </underlyingSecurityTitle>
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                </underlyingSecurityShares>
            </underlyingSecurity>
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        <derivativeTransaction>
            <securityTitle>
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                <value>13.47</value>
            </conversionOrExercisePrice>
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                <equitySwapInvolved>false</equitySwapInvolved>
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    </derivativeTable>

    <footnotes>
        <footnote id="F1">Represents restricted stock units award granted under the Issuer's 2024 Equity Incentive Plan (&quot;RSUs&quot;).</footnote>
        <footnote id="F2">On December 12, 2025, the Issuer cancelled, pursuant to an option exchange, the options listed in Table II. In exchange for the cancelled options, the Reporting Person received the RSUs reported in Table I.</footnote>
        <footnote id="F3">25% of the shares vested on October 22, 2021, and the remaining shares vest in 36 equal monthly installments thereafter.</footnote>
        <footnote id="F4">25% of the shares vested on January 1, 2024, and the remaining shares vest in 36 equal monthly installments thereafter.</footnote>
        <footnote id="F5">25% of the shares vested on February 22, 2022, and the remaining shares vest in 36 equal monthly installments thereafter.</footnote>
        <footnote id="F6">Vesting is monthly over a 48-month period starting January 1, 2024 in equal monthly amounts.</footnote>
        <footnote id="F7">25% of the shares will vest on July 19, 2025, and the remaining shares vest in 36 equal monthly installments thereafter.</footnote>
    </footnotes>

    <ownerSignature>
        <signatureName>/s/ Andrew Cronauer, Attorney-in-Fact</signatureName>
        <signatureDate>2025-12-12</signatureDate>
    </ownerSignature>
</ownershipDocument>
