<?xml version="1.0"?>
<ownershipDocument>

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    <periodOfReport>2026-10-13</periodOfReport>

    <notSubjectToSection16>0</notSubjectToSection16>

    <issuer>
        <issuerCik>0001512922</issuerCik>
        <issuerName>PetVivo Holdings, Inc.</issuerName>
        <issuerTradingSymbol>PETV</issuerTradingSymbol>
        <issuerForeignTradingSymbol></issuerForeignTradingSymbol>
    </issuer>

    <reportingOwner>
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            <rptOwnerName>A.L. Sarroff Fund, LLC</rptOwnerName>
        </reportingOwnerId>
        <reportingOwnerAddress>
            <rptOwnerNonUSAddressFlag>false</rptOwnerNonUSAddressFlag>
            <rptOwnerStreet1>43 MEADOW WOODS ROAD</rptOwnerStreet1>
            <rptOwnerStreet2></rptOwnerStreet2>
            <rptOwnerCity>GREAT NECK</rptOwnerCity>
            <rptOwnerState>NY</rptOwnerState>
            <rptOwnerZipCode>11020</rptOwnerZipCode>
            <rptOwnerStateDescription></rptOwnerStateDescription>
        </reportingOwnerAddress>
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            <isOfficer>0</isOfficer>
            <isTenPercentOwner>1</isTenPercentOwner>
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            <officerTitle></officerTitle>
            <otherText></otherText>
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                    <value>0.70</value>
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                    <value>0.7487</value>
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    <footnotes>
        <footnote id="F1">Alan L. Sarroff is the Chief Executive Officer and Managing Member of A.L. Sarroff Fund, LLC and is deemed to beneficially own and have sole voting and dispositive powers over its securities.</footnote>
        <footnote id="F2">Held by Mr. Sarnoff's spouse, Eileen Sarroff.</footnote>
    </footnotes>

    <ownerSignature>
        <signatureName>/s/ A.L. Sarroff Fund, LLC, By: Alan L. Sarroff, Chief Executive Officer and Managing Member</signatureName>
        <signatureDate>2026-10-21</signatureDate>
    </ownerSignature>
</ownershipDocument>
