<?xml version="1.0"?>
<ownershipDocument>

    <schemaVersion>X0508</schemaVersion>

    <documentType>4</documentType>

    <periodOfReport>2024-10-18</periodOfReport>

    <notSubjectToSection16>0</notSubjectToSection16>

    <issuer>
        <issuerCik>0001512922</issuerCik>
        <issuerName>PetVivo Holdings, Inc.</issuerName>
        <issuerTradingSymbol>PETV</issuerTradingSymbol>
    </issuer>

    <reportingOwner>
        <reportingOwnerId>
            <rptOwnerCik>0001553209</rptOwnerCik>
            <rptOwnerName>A.L. Sarroff Fund, LLC</rptOwnerName>
        </reportingOwnerId>
        <reportingOwnerAddress>
            <rptOwnerStreet1>43 MEADOW WOODS ROAD</rptOwnerStreet1>
            <rptOwnerStreet2></rptOwnerStreet2>
            <rptOwnerCity>GREAT NECK</rptOwnerCity>
            <rptOwnerState>NY</rptOwnerState>
            <rptOwnerZipCode>11020</rptOwnerZipCode>
            <rptOwnerStateDescription></rptOwnerStateDescription>
        </reportingOwnerAddress>
        <reportingOwnerRelationship>
            <isDirector>0</isDirector>
            <isOfficer>0</isOfficer>
            <isTenPercentOwner>1</isTenPercentOwner>
            <isOther>0</isOther>
            <officerTitle></officerTitle>
            <otherText></otherText>
        </reportingOwnerRelationship>
    </reportingOwner>

    <aff10b5One>0</aff10b5One>

    <nonDerivativeTable>
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            <securityTitle>
                <value>Common Stock</value>
            </securityTitle>
            <transactionDate>
                <value>2024-10-18</value>
            </transactionDate>
            <deemedExecutionDate></deemedExecutionDate>
            <transactionCoding>
                <transactionFormType>4</transactionFormType>
                <transactionCode>P</transactionCode>
                <equitySwapInvolved>0</equitySwapInvolved>
            </transactionCoding>
            <transactionTimeliness></transactionTimeliness>
            <transactionAmounts>
                <transactionShares>
                    <value>7501</value>
                </transactionShares>
                <transactionPricePerShare>
                    <value>0.52</value>
                </transactionPricePerShare>
                <transactionAcquiredDisposedCode>
                    <value>A</value>
                </transactionAcquiredDisposedCode>
            </transactionAmounts>
            <postTransactionAmounts>
                <sharesOwnedFollowingTransaction>
                    <value>6618431</value>
                </sharesOwnedFollowingTransaction>
            </postTransactionAmounts>
            <ownershipNature>
                <directOrIndirectOwnership>
                    <value>D</value>
                    <footnoteId id="F1"/>
                </directOrIndirectOwnership>
            </ownershipNature>
        </nonDerivativeTransaction>
        <nonDerivativeTransaction>
            <securityTitle>
                <value>Common Stock</value>
            </securityTitle>
            <transactionDate>
                <value>2024-10-22</value>
            </transactionDate>
            <deemedExecutionDate></deemedExecutionDate>
            <transactionCoding>
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                <transactionCode>P</transactionCode>
                <equitySwapInvolved>0</equitySwapInvolved>
            </transactionCoding>
            <transactionTimeliness></transactionTimeliness>
            <transactionAmounts>
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                </transactionShares>
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                </transactionAcquiredDisposedCode>
            </transactionAmounts>
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            <ownershipNature>
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            </ownershipNature>
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            </securityTitle>
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            </transactionDate>
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            <transactionTimeliness></transactionTimeliness>
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                </transactionShares>
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                </transactionPricePerShare>
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                </transactionShares>
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            </securityTitle>
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        </nonDerivativeTransaction>
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            </securityTitle>
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                <equitySwapInvolved>0</equitySwapInvolved>
            </transactionCoding>
            <transactionTimeliness></transactionTimeliness>
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                </transactionShares>
                <transactionPricePerShare>
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                </transactionPricePerShare>
                <transactionAcquiredDisposedCode>
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                </sharesOwnedFollowingTransaction>
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            </ownershipNature>
        </nonDerivativeTransaction>
        <nonDerivativeTransaction>
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                <value>Common Stock</value>
            </securityTitle>
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            </transactionDate>
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                </transactionShares>
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                </transactionPricePerShare>
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            </transactionAmounts>
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                <sharesOwnedFollowingTransaction>
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                </sharesOwnedFollowingTransaction>
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                <directOrIndirectOwnership>
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        <nonDerivativeTransaction>
            <securityTitle>
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            </securityTitle>
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                    <value>15001</value>
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                <sharesOwnedFollowingTransaction>
                    <value>7398774</value>
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                <sharesOwnedFollowingTransaction>
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                    <value>7508564</value>
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                    <value>0.55</value>
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                    <value>A</value>
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                    <value>7509564</value>
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                <transactionShares>
                    <value>215014</value>
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                    <value>0.47</value>
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                    <value>A</value>
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                    <value>7724578</value>
                </sharesOwnedFollowingTransaction>
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                    <value>D</value>
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            <securityTitle>
                <value>Common Stock</value>
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                <transactionFormType>4</transactionFormType>
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                <transactionShares>
                    <value>16001</value>
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                <transactionPricePerShare>
                    <value>0.57</value>
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                <transactionAcquiredDisposedCode>
                    <value>A</value>
                </transactionAcquiredDisposedCode>
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                <sharesOwnedFollowingTransaction>
                    <value>7740579</value>
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                <directOrIndirectOwnership>
                    <value>D</value>
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    </nonDerivativeTable>

    <footnotes>
        <footnote id="F1">Alan L. Sarroff is the Chief Executive Officer and Managing Member of A.L. Sarroff Fund, LLC and is deemed to beneficially own and have sole voting and dispositive powers over its securities.</footnote>
    </footnotes>

    <ownerSignature>
        <signatureName>/s/ A.L. Sarroff Fund, LLC, By: Alan L. Sarroff, Chief Executive Officer and Managing Member</signatureName>
        <signatureDate>2026-01-16</signatureDate>
    </ownerSignature>
</ownershipDocument>
